Road Ecology has emerged as a transdisciplinary field of applied research in response to continuous increase in transportation infrastructure worldwide. Road network connects cities and villages, agricultural and natural areas and influence the landscape in various ways. Roads and traffic alter the environment polluting air and water and affecting not only wildlife communities but also human well-being. Road ecology addresses human security and animal road kill mitigation, while maintaining roads functionality. Our research team has adapted habitat suitability models to identify road kill probability along roads in response to landscape attributes. We also investigate how road upgrading may affect the spatial distribution of wildlife road mortality.